Most 19th century Czech migrants to today’s Ukraine settled in the agriculturally potent western and southern regions. However, a large number of these migrants found employment also in the then developing urban centres. Odessa, now a port metropolis located in the southwestern part of Ukraine, was one of such cities wherein communities of Czech compatriots gradually established. The article considers the presence of the Czech minority in the city of Odessa, emphasizing a its communal and social life. The study also recalls probably the most important figure of Czech origin in the field of culture, the long-time chief conductor of the local city theatre orchestra and composer Josef Přibík.